Protocols

Actin Cosedimentation
Adjusting the hot Bonnet's lid pressure (MJR)
Bio-Rad DEAE Aff-Gel Blue Gel Instruction Manual (PDF)
Bio-Rad Protein Assay Manual (PDF)
Bradford Microassay Procedure (protein quantitation)
Colony PCR
Conversions
Coupling a Peptide to Affi-Gel-10 Agarose
Designing a new program (MJR)
DNA Precipitation
DNA Quantitation (Biocrom Ultrospec 2000)
Double Sequential
Fluorochromes and Filters (PDF)
Frozen Stocks of Bacteria and Yeast (Dr. J. Ngsee's Protocol)
Gelatin-coating of glass coverslips
GST fusion protein purification
His-Tag Fusion Protein Purification
Immunohisochemical Procedures
Immunoprecipitation
Induction of DE3 Lysogens
Introduction to Epitope Tagging (PDF, pages 1-2, 3-4, 5-8, 8-10, 11-12, 13-16, 16-17, 18-19, 20, 21-22 ,23-24)
Lynn's Awesome Tissue Staining Protocol
Membrane Extraction
Millipore Ultrafilters Manual (PDF)
Notes from Confocal Microscope
Plasmid DNA Rapid Maxi-Prep
Precipitation of Ligation Reactions
Preparing LB agar plates
Preparing Glycerol Stocks of Bacterial Cultures
Preparing Thioredoxin Fusion Proteins
PVDF membranes
QIAEX II Agarose Gel Extraction
QIAGEN Plasmid Purification: Midi and Maxi kits
QIAquick PCR Purification Kit
SDS-Page And Westerns
SOC Medium
Standardize the pH meter
Torpedo Membrane Preparation - Band 2 
Transfection Protocol (Calcium Phosphate method)
Transformation of Competent cells
Westerns
X-Gal Plates