The zebrafish laboratory is located in the Center for Applied Research in Environmental Genomics (CAREG).
CAREG is part of the University of Ottawa - New Biosciences Complex.
The zebrafish laboratory is located in the Center for Applied Research in Environmental Genomics (CAREG).
CAREG is part of the University of Ottawa - New Biosciences Complex. |
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The microinjection workstation consists of a Narishige microinjector/micromanipulation system and a Nikon SMZ-1500 microscope equipt with OCC illumination base and epifluorescence illuminator. Using this apparatus we are able to microinject plasmid DNA constructs into fertilized zebrafish eggs in order to produce transgenic zebrafish. Antisense oligonucleotides can be microinjected into one cell zebrafish embryos to produce embryos deficient in a particular protein. |
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| In situ hybridization is a process that allows the labelling of cells within a zebrafish embryo that expess a specific gene. Using the automated in situ hybridization system shown here, we are able to conduct 100 whole mount in situ hybridization reactions in approximately 24 hours. |  |
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| A glimpse inside the Zebrafish Lab, which is located in room 323 in CAREG. To your right are the PCR machines of the laboratory. |  |
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